Journal: Cell reports

Article Title: History-dependent dopamine release increases cAMP levels in most basal amygdala glutamatergic neurons to control learning

doi: 10.1016/j.celrep.2022.110297

Figure Lengend Snippet: Key resources table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Virus strains AAV2/1.hSyn.FLEX.GCaMP6s.WPRE Addgene CAT# 100845 AAV2/1.hSyn.DIO.GreenDownward-cADDis.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/1.hSyn-dLight1.1.WPRE Boston Children’s Hospital Viral Core Lutas et al., 2019 AAV2/1.EF1a.DIO.mKate2-biPAC.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/1.EF1a.DIO.mKate2-PDE4D3-cat.WPRE Boston Children’s Hospital Viral Core Zhang et al., 2021b AAV2/9.hSyn.FLEX.ExRai-AKAR2-CW3SL Richard Huganir Zhang et al., 2021a AAV2/1.hSyn.FLEX.Chrimson-tdTomato UNC Vector Core Chemicals, peptides, and recombinant proteins SCH23390 hydrochloride Tocris Cat# 0925 {"type":"entrez-protein","attrs":{"text":"SKF81297","term_id":"1156277425","term_text":"SKF81297"}} SKF81297 hydrobromide Tocris Cat# 1447 Dopamine hydrochloride Tocris Cat# 3548 Forskolin Tocris Cat# 1099 IBMX Tocris Cat# 2845 Experimental models: Organisms/strains Mouse: EMX1-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:005628 Mouse: DAT-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:020080 Software and algorithms MATLAB R2019 Mathworks https://www.mathworks.com/products/matlab.html; RRID: SCR_001622 ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Scanbox Neurolabware https://scanbox.org/ Other 10x, 0.5 NA objective ThorLabs CAT# TL10X-2P 4x, 0.28 NA objective Olympus XLFUOR 16x, 0.8 NA objective Nikon CFI75 Two-photon microscope Neurolabware http://neurolabware.com/ InSight X3 laser Spectra-Physics InSight X3 Open in a separate window Key resources table Basal amygdala cAMP signaling facilitates learning of motivational salience of cues Axonal dopamine release increases cAMP in most basal amygdala glutamatergic neurons Presynaptic depression of dopamine release limits postsynaptic cAMP accumulation cAMP responses depress following clustered appetitive or aversive stimuli

Techniques: Plasmid Preparation, Recombinant, Software, Microscopy

Journal: Scientific Reports

Article Title: Sierra Nevada sweep: metagenomic measurements of bioaerosols vertically distributed across the troposphere

doi: 10.1038/s41598-020-69188-4

Figure Lengend Snippet: Sampling over the US Sierra Nevada mountain range. ( a ) Aircraft Bioaerosol Collector (ABC) onboard NASA’s C-20A aircraft. Air sampling line, flow valve, cascade impactor, and air flow meter shown on workbench; ( b ) June 20, 2018, view from C-20A window at lowest altitude sampled (10,000 ft) above the US Sierra Nevada mountain range with an aerosol haze visible on the horizon; ( c ) Flight pattern on June 20, 2018 based on GPS data, sampling for 30–40 min at 10,000 ft steps up to 40,000 ft; ( d ) Flight pattern on June 21, 2018 based on GPS data, sampling for 30–40 min starting at 40,000 ft and stepping down in 10,000 ft steps. Panels C and D were generated using Mathworks Matlab software, versions R2018 to R2019 ( https://www.mathworks.com/products/matlab.html ), from the recorded flight path.

Article Snippet: Panels were generated using Mathworks Matlab software, versions R2018 to R2019 ( https://www.mathworks.com/products/matlab.html ), from the modelling described in the text.

Techniques: Sampling, Aerosol, Generated, Software

Journal: Scientific Reports

Article Title: Sierra Nevada sweep: metagenomic measurements of bioaerosols vertically distributed across the troposphere

doi: 10.1038/s41598-020-69188-4

Figure Lengend Snippet: Aerosol dispersal predictions. ( a ) 7-day model forecast (June 20, 2018 to June 27, 2018) with grey contours showing fraction of sampled material that could have been inhaled by an average outdoor individual (individual exposure potential); ( b ) The blue contours show the population density (people per sq km) in the modeling domain; ( c ) The blue-grey contours show the population level exposure potential derived by combining individual exposure potential (shown in a ) with population estimates (shown in b ). This image shows geographic distribution of potentially inhaled material (inhaled fraction of sample material per sq km). Panels were generated using Mathworks Matlab software, versions R2018 to R2019 ( https://www.mathworks.com/products/matlab.html ), from the modelling described in the text. Underlying map data was provided by Google and INEGI and accessed through the Google Maps Platform API.

Article Snippet: Panels were generated using Mathworks Matlab software, versions R2018 to R2019 ( https://www.mathworks.com/products/matlab.html ), from the modelling described in the text.

Techniques: Aerosol, Derivative Assay, Generated, Software

Journal: Cells

Article Title: Melatonin, a Potential Therapeutic Agent for Preeclampsia, Reduces the Extrusion of Toxic Extracellular Vesicles from Preeclamptic Placentae

doi: 10.3390/cells10081904

Figure Lengend Snippet: The misfolded proteins in placental micro-EVs ( A ) and nano-EVs ( B ) collected from either preeclamptic (PE) or normotensive placentae were measured by ThT fluorescent intensity. The statistical analysis was assessed by an ANOVA test or a Mann–Whitney U-test, when appropriate.

Article Snippet: The fluorescent density of each line on the images was calculated by Matlab software (R2019 A version).

Techniques: MANN-WHITNEY

Journal: Cells

Article Title: Melatonin, a Potential Therapeutic Agent for Preeclampsia, Reduces the Extrusion of Toxic Extracellular Vesicles from Preeclamptic Placentae

doi: 10.3390/cells10081904

Figure Lengend Snippet: The levels of misfolded proteins in preeclamptic placentae, especially in the syncytiotrophoblast ( A ) and in normotensive term placentae ( B ), were measured by ThT fluorescent intensity. The statistical difference was confirmed by a semi-quantitative assay ( C ).

Article Snippet: The fluorescent density of each line on the images was calculated by Matlab software (R2019 A version).

Techniques:

Journal: Cells

Article Title: Melatonin, a Potential Therapeutic Agent for Preeclampsia, Reduces the Extrusion of Toxic Extracellular Vesicles from Preeclamptic Placentae

doi: 10.3390/cells10081904

Figure Lengend Snippet: Misfolded proteins in preeclamptic placental explants (PE) that had been treated with 1 µM ( B ) or 10 µM ( C ) melatonin or without melatonin ( A ) were measured by the fluorescent intensity of ThT. In comparison, misfold proteins in normotensive placental explants that had been treated with 10 µM ( D ) were also measured. The statistical difference was confirmed by a semi-quantitative assessment and assessed by an ANOVA test ( E ).

Article Snippet: The fluorescent density of each line on the images was calculated by Matlab software (R2019 A version).

Techniques: Comparison